Sheffield Institute for Nucleic Acids

Dr Alice Pyne

Research interests

Our research looks to determine how variations in DNA structure affect fundamental biological processes including replication and transcription. This research is facilitated by atomic force microscopy (AFM) methods, developed in collaboration with industry, capable of resolving the double-helical structure of single DNA molecules. In addition, we develop quantitative, open-source analysis tools that allow us to exploit our large datasets in order to acquire a unique perspective on biomolecular structure and function.
These high resolution methods allow us to determine how DNA structure, conformation and dynamics vary at the level of single biomolecules. These variations, only observable when molecules are probed on these time and length scales, provide unique insights into how the complexity of DNA structure relates to its biological function.